The decreased levels of N-acetyl serine could be an indication for protein degradation. anti-metastatic potential. Those significant anti-motility properties were verified by a Mitoxantrone reduced ability for migration (60%), invasion (99%) and colony formation (61%) under 48?h exposure to 25?g/ml VSM. The restricted motility features were due to an induction of the stabilization of the cytoskeleton C actin materials were 2.5-fold longer and were spanning the entire cell. Decreased proliferation (PCNA, AMT, GCSH) and modified metastasis (e. g. SGPL1, CXCR4, stathmin) marker manifestation on transcript and protein level confirmed the significant lowered tumorigenicity under VSM treatment. Finally, significant alterations in the cell rate of metabolism were recognized for 25 metabolites, with levels of uracil, N-acetyl serine and propanoyl phosphate harboring the greatest alterations. Compared to the standard therapy with cisplatin, VSM treated cells shown a similar metabolic shutdown of the primary cell metabolism. Main control cells were not affected by the VSM treatment. Conclusions This study exposed the VSM root extract like a potential, new migrastatic drug candidate for the putative treatment of pediatric alveolar rhabdomyosarcoma with actin filament stabilizing properties and accompanied by a marginal effect on the vitality of main cells. Graphical abstract Supplementary Info The online version consists of supplementary material available at 10.1186/s12906-021-03299-x. (VSM; methanol whole flower draw out) and (LW; ethanol root extract) were evaluated. The main effective elements of Linum were already analyzed by mass spectrometry in one of our former projects . We primarily recognized isoflavones and lignans, for example secoisolariciresinol, matairesinol, biochanin, daidzein and glycites – classical phytoestrogens, whose anti-cancer effects have been verified. Therefore, selected solitary phytoestrogens, abbreviated as secoisolariciresinol (Seco), matairesinol (Mata), daidzein (Daid) and genistein (Geni) were evaluated within the metastasizing property of alveolar RMS cells. The secondary flower compounds Seco, Mata, Daid are known to reduce tumor growth of breast and prostate malignancy cells [22C26]. The secondary flower substance Geni is definitely a main Mitoxantrone ingredient of soy (Wight (VSM) flower extract was provided by Dr. Iftikhar Ali (Karakoram International University or college, Division of Chemistry, Gilgit, Pakistan) and its collection and recognition were explained previously . Briefly, it was collected from Baluchistan (Pakistan) and its authentication was carried out from the flower taxonomists Prof. Rasool Bakhsh Tareen (Division of Botany, University or college of Balochistan, Quetta, Pakistan) and Dr. Sher Wali Khan (Division of Biological Sciences, Karakoram International University Mitoxantrone Mitoxantrone or college, Gilgit, Pakistan). The VSM flower sample was extracted in MeOH and 50?mg of its dry sample was dissolved in 1?ml DMSO to a 50?mg/ml stock solution. The (LW) flower material and extract preparation from native flax origins was explained previously Mitoxantrone . Briefly, the seeds were from the Agriculture Study Institution (LUFA, Rostock, Germany) and the vegetation were raised in the University or college of Rostock (Rostock, Germany). The dry extract powder was dissolved in EtOH to a stock remedy of 100?mg/ml. The stock solutions of plant-derived components were further diluted in different concentrations for the biological investigation and anti-tumor activity screening. The main secondary flower compounds of (VSM) and L. (LW) were previously recognized by NMR and LC-MS analysis in accordance to their retention time [22, 29] and outlined in Table?1. Table 1 Main secondary flower compounds of (VSM) and L. by NMR and LC-MS analysis in accordance to their retention time [22, 29] (VSM)L. (LW)ideals: *** Wight and and four plant-derived compounds: genistein, daidzein, matairesinol and secoisolariciresinol within the MKI67 pediatric RMA cell collection RH-30 [21C23]. The initial testing exposed the VSM extract as the most potential anti-metastatic agent illustrated from the significant reduction of the migration, invasion, and colony formation in RH-30 cells (Fig..