The next primary antibodies were used: rabbit anti-horseradish peroxidase (HRP) (1:500), mouse anti-Elav (1:10), mouse anti-BP104 (1:10), mouse anti-Prospero (1:10), mouse anti-cyclin A (through the Developmental Research Hybridoma Standard bank, Iowa city, Iowa), mouse anti-Worniu (1:1000) kindly supplied by Dr

The next primary antibodies were used: rabbit anti-horseradish peroxidase (HRP) (1:500), mouse anti-Elav (1:10), mouse anti-BP104 (1:10), mouse anti-Prospero (1:10),…

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Nissl-stained histological sections revealed that cKO embryos displayed a dysgenesis of the olfactory bulbs (data not shown) and SE, including an almost complete absence of the midline (15% penetrance, n = 25) that resulted in a mild rostral holoprosencephaly (Figure 1e, e’, e”; Additional data file 2)

Nissl-stained histological sections revealed that cKO embryos displayed a dysgenesis of the olfactory bulbs (data not shown) and SE, including…

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